Evaluation of laparoscopic resection of colorectal carcinoma from the viewpoint of molecular biology

نویسندگان

  • Matej Skrovina
  • Miloslav Duda
  • Josef Srovnal
  • Jiri Bartos
  • Lenka Radova
  • Marian Hajduch
  • Renata Soumarova
چکیده

AIM Detection of the possible impact of surgical management on the occurrence of minimal residual disease (MRD) in patients with colorectal carcinoma (CRC) in bone marrow samples, portal and peripheral blood samples. Comparison of MRD prevalence in patients with laparoscopic resection of CRC and those with open colorectal resection. Assessment of the potential impact of MRD on the relapse of the disease and overall survival of patients. MATERIAL AND METHODS The study included 124 patients with primary CRC without proven distant metastases indicated for elective laparoscopic resection and operated on between September 21, 2006 and December 31, 2008 at the Department of Surgery, Hospital and J.G. Mendel Oncological Centre Novy Jicin. 6 samples were collected from each patient to determine MRD (system venous blood and bone marrow at the beginning of surgery, venous blood from mesenteric bloodstream, system venous blood after the resection, system venous blood and bone marrow 1 month after the surgery). Detection of MRD on the basis of CEA expression was performed by real-time RT-PCR technique. The results were compared with those obtained within the similar research using the same methodology at the 2(nd) Department of Surgery, University Hospital in Olomouc (the group included 230 patients treated with open resection of CRC). RESULTS In the group of patients treated with laparoscopic resection, a correlation between positive MRD in the sample of bone marrow collected before the surgery and the stage of the disease was found (p < 0.035). We also recorded the impact of surgical management on MRD occurrence in system venous blood in primary negative patients (p < 0.025). However, in the course of the short period of time we have not found a statistically significant correlation between the finding in patients with stage I-III, and the period prior to the relapse of the disease (p < 0.59). With regard to the results obtained, we can expect a potential direct correlation between a positive MRD finding in system venous blood taken prior to surgery in patients with stage I-III CRC and shorter time of survival (p < 0.075). In the group of patients treated with open resection of CRC, no statistically significant relationship between the stage of the disease and MRD occurrence was found. Incidence of circulating tumour cells (CTC) in the samples of venous blood taken prior to surgery is a prognostically important factor (p < 0.05) from the viewpoint of disease-free survival (DFS). Another prognostically important factor with regard to DFS seems to be the occurrence of disseminated tumour cells (DTC) in the bone marrow taken 1 month after the surgery. CONCLUSIONS The data recorded suggest a relationship between MRD finding and the disease prognosis. Collection of samples as well as evaluation of results continue as we strive to include more patients in our study and to obtain more data within 5-10 years of the follow-up. The comparison between the data obtained in the laparoscopic approach and the data obtained in open resection performed from the viewpoint of molecular biology did not show a significant difference in MRD detection in the samples collected after the surgery.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Laparoscopic Colon Surgery for Benign Disease: A Comparison to Open Surgery

Backgrounds and Objectives: Today we are witnessed a growing number of minimally invasive surgical techniques for different diseases. Laparoscopic colon surgery as a minimally invasive surgery is currently growing in treatment of malignancies after proving his place in the treatment of benign diseases. We compare in this study results of laparoscopic colorectal surgery with open surgery. Method...

متن کامل

SYNCHRONOUS SURGICAL REMOVAL OF SUSPICIOUS OVARIAN METASTASES FROM COLORECTAL CANCER

Metastatic ovarian cancers occur in 3-8 percent of women undergoing surgical resection of a primary colorectal cancer. In this study we examined the characteristics of women with colorectal cancer for whom synchronous oophorectomy had been performed. In this cross-sectional study, records of patients with colorectal cancer from April 1991 through October 1999 who had undergone surgery for ...

متن کامل

Epigenetics in diagnosis of colorectal cancer

Colorectal cancer (CRC) is a third most common epithelial carcinoma. CRC is known to develop from the early precancerous lesion to full blown malignancy via definite phases due to cumulative mutations and aberrant methylation of number of genes. The use of serum biomarkers that is non-invasive to discriminate cancer patients from healthy persons will prove to be an important tool to improve the...

متن کامل

Effects of colorectal endoscopic submucosal dissection on treatment for Tis carcinoma

Objective: From the viewpoint of surgery, this study examined the effects of colorectal endoscopic submucosal dissection (ESD) on treatment for Tis carcinoma over the past 10 years since the technique was adopted. Materials and methods: Among the 3,826 colorectal cancer patients treated at Juntendo University within the 20-year period between 1996 and 2015. The total number of colorectal ESD pe...

متن کامل

SYNCHRONOUS SURGICAL REMOVAL OF SUSPICIOUS OVARIAN METASTASES FROM COLO RECTAL CANCER

 Metastatic ovarian cancers occur in 3-8 percent of women undergoing surgical resection of a primary colorectal cancer. In this study we examined the characteristics of women with colorectal cancer for whom synchronous oophorectomy had been performed. In this cross-sectional study, records of patients with colorectal cancer from April 1991 through October 1999 who had undergone surgery for both...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 7  شماره 

صفحات  -

تاریخ انتشار 2012